Segment nuclei in 2D images by Laplacian of Gaussian (user defined radius) and thresholding (user defined level) followed by binary watershed transform.
This workflow is described in www.biii.eu in http://biii.eu/nuclei-segmentation-2d-imagej
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BIAFLOWS workflow to segment clustered nuclei using a laplacian filter, thresholding and a binary watershed transform.
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