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This has probably already been asked before, but I can't find the right answer. I'm running the Seurat v5 integration workflow and would like to visualize some genes after the integration.
Seurat object before the integration contains CD79A gene:
An object of class Seurat
28856 features across 4792 samples within 2 assays
Active assay: SCT (11996 features, 3000 variable features)
3 layers present: counts, data, scale.data
1 other assay present: RNA
3 dimensional reductions calculated: pca, integrated.dr, umap
Now I try to plot CD79A expression:
FeaturePlot(seurat_obj, features = c('CD79A'))
And get the error:
Warning message:
“No layers found matching search pattern provided”
Warning message:
“The following requested variables were not found: CD79A”
Error in `FeaturePlot()`:
! None of the requested features were found: CD79A in slot data
Traceback:
1. abort(message = paste("None of the requested features were found:",
. paste(features, collapse = ", "), "in slot ", slot))
2. signal_abort(cnd, .file)
3. signalCondition(cnd)
I tied setting return.only.var.genes = F to return all genes, but it doesn't help. What am I missing?
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This has probably already been asked before, but I can't find the right answer. I'm running the Seurat v5 integration workflow and would like to visualize some genes after the integration.
Seurat object before the integration contains CD79A gene:
I ran the integration:
The resulting object:
Now I try to plot CD79A expression:
And get the error:
I tied setting
return.only.var.genes = F
to return all genes, but it doesn't help. What am I missing?Beta Was this translation helpful? Give feedback.
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