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seppinho committed Nov 16, 2018
2 parents 2ac5390 + 6a6f69a commit 26e0366
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Expand Up @@ -5,20 +5,23 @@ Mutation-Server is a Java library to detect heteroplasmic and homoplasmic sites
It has been integrated in [mtDNA-Server](https://mtdna-server.uibk.ac.at). For scalability, Mutation-Server is parallelized using Hadoop MapReduce but also available as a standalone tool.

## Standalone Usage
You can run Mutation-Server as a standalone tool starting with CRAM/BAM files and detecting heteroplasmic and homoplasmic sites. By default BAQ is set (``--noBaq`` otherwise) and no indels (beta feature, ``--indel`` otherwise) are called. Please note that currently only deletions are included when activating ``--indel``.
You can run Mutation-Server as a standalone tool starting with CRAM/BAM files and detecting heteroplasmic and homoplasmic sites. By default BAQ is set (``--noBaq`` otherwise) and no indels (``--indel`` otherwise) are called. Please note that currently **only deletions** are included when activating ``--indel``.
```
wget https://github.com/seppinho/mutation-server/releases/download/v1.1.10/mutation-server-1.1.10.jar
wget https://github.com/seppinho/mutation-server/releases/download/v1.1.11/mutation-server-1.1.11.jar
java -jar mutation-server-1.1.10.jar analyse-local --input <file/folder> --output <folder> --reference <fasta> --level 0.01
java -jar mutation-server-1.1.11.jar analyse-local --input <file/folder> --output <folder> --reference <fasta> --level 0.01 --writeVcf
```

### Default parameter
### Default Parameters

| Parameter | Value |
| ------------- |:-------------:|
| MappingQuality | 20 |
| BaseQuality | 20 |
| AlignmentQuality | 20 |
| Parameter | Value | Command Line Option |
| ------------- |:-------------:| :-------------:|
| MappingQuality | 20 | `--mapQ`|
| BaseQuality | 20 | `--baseQ`|
| AlignmentQuality | 30 | `--alignQ`|
| noBAQ | - | `--noBAQ`|
| Write VCF | - | `--writeVcf`|
| Write raw | - | `--writeRaw`|


## Output Formats
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