neurolabware_scanbox_utils_docs

Collected code, issues and experiences about the Neurolabware Mesoscope multiphoton imaging platform and ScanBox software

Official docs

• http://neurolabware.com/
• https://scanbox.org/ - incomplete and based on some previous version of ScanBox

Known issues with official ScanBox software / Mesoscope hardware

• FIXED: Occasionally, objective moves to some far-away position after end of focus
• Optotune scheduling for z-stacks of mesoscopic panoramas not working: Optotune doesn't move
• When turning Knobby control knobs fast in "Coarse" control mode, motion doesn't stop as soon as control knob rotation is stopped
• FIXED: In mesoscopic panorama display window, accumulation can't be disabled, which would be required for acquisitions other than time series that don't include motor or remote focus motion
• See NLW_mesoscope_wisdom.pptx for a lot more bugs and usability issues

Collected wisdom

• There is no overcurrent protection of the PMTs, so the user has to make sure that bright lights are off during all acquisitions
• There is no widefield observation capability, which makes sample positioning and focus finding hard
• See NLW_mesoscope_wisdom.pptx for collected recipes and errors