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Casting Light on Chromosomes: A Study of FISH and DAPI Image Segmentation in Cancer Research

This contains the code for our chromosome segmentation tool. The notebook files were for our personal testing and early implemntations.

Directory

File Description
config.ini Configuration file to set parameters
environment.yml File to create the environment
Folder Description
sample Folder containing sample images to use with our project
src Contains our Python scripts

Installation

To get started, run the following code to create the environment.

git clone 
cd ecDNA-Capstone
conda env create -f environment.yml
conda activate cap

Image Specifications

Input folder will only read .tif files

Instructions

  1. Change the config.ini file to your own file path. If not set, 3 sample images are provided to test output.
  2. Run chromosome.py
  3. Annotated images will show up under annotated/filename/a_filename.tif
  4. output.csv contains info in the format of filename, number of chromosomes

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Chromosome Segmentation Tool

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