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Populate tob-wgs-test metamist with nagim gvcf files #653

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a1b5be9
Merge pull request #574 from populationgenomics/dev
illusional Oct 12, 2023
4cb1744
Merge pull request #579 from populationgenomics/dev
illusional Oct 19, 2023
6d77327
Merge pull request #581 from populationgenomics/dev
illusional Oct 21, 2023
cb53231
Merge pull request #584 from populationgenomics/dev
illusional Oct 23, 2023
24ae802
Merge pull request #587 from populationgenomics/dev
illusional Oct 25, 2023
37618f1
Merge pull request #595 from populationgenomics/dev
illusional Oct 31, 2023
f222398
Merge pull request #599 from populationgenomics/dev
illusional Nov 1, 2023
e8025df
Merge pull request #613 from populationgenomics/dev
vivbak Nov 15, 2023
f8acb63
Billing pre-release (#605) (#617)
milo-hyben Nov 20, 2023
a684651
Merge pull request #618 from populationgenomics/dev
vivbak Nov 21, 2023
7db6896
Merge pull request #623 from populationgenomics/dev
vivbak Nov 23, 2023
5beb48d
Merge pull request #632 from populationgenomics/dev
michael-harper Dec 11, 2023
2a49cd9
Merge pull request #639 from populationgenomics/dev
illusional Jan 3, 2024
570809a
Merge pull request #641 from populationgenomics/dev
illusional Jan 5, 2024
3d2df80
Merge pull request #644 from populationgenomics/dev
illusional Jan 9, 2024
1a2f22a
Merge pull request #649 from populationgenomics/dev
nevoodoo Jan 11, 2024
6f619d0
script to create samples into tob-wgs metamist
michael-harper Jan 15, 2024
2645935
changed how to read path
michael-harper Jan 15, 2024
b97ecd2
changed project id to be an integer
michael-harper Jan 15, 2024
7f963b7
making assays not None
michael-harper Jan 15, 2024
db607f2
assays has to be a list
michael-harper Jan 15, 2024
c896d30
adding assay meta fields
michael-harper Jan 15, 2024
beb62f1
added script to add gvcf analysis entries metamist
michael-harper Jan 15, 2024
57e8e83
fixed variable naming
michael-harper Jan 15, 2024
ce4f7b9
changed analysis status from COMPLETED to completed
michael-harper Jan 15, 2024
fef13dc
removed unnecessary fields from analysis upsert
michael-harper Jan 15, 2024
c1e1b7e
now adding analyses to correct sequencingGroups
michael-harper Jan 15, 2024
b7ba499
generating participants
michael-harper Jan 16, 2024
3c131d8
importing and global variable changes
michael-harper Jan 16, 2024
7ee6d51
adding necessary assay upsert fields
michael-harper Jan 16, 2024
fb2c690
adding more required fields to assay upsert
michael-harper Jan 16, 2024
225f6fd
editing assays so that their "type" is unique
michael-harper Jan 16, 2024
8196100
adding new participants to tob-wgs-test metamist
michael-harper Jan 17, 2024
2bf2f65
removed hard coding of mapping file
michael-harper Jan 17, 2024
b46e8f8
remove unwanted files
michael-harper Jan 17, 2024
76eb16f
making suggested changes
michael-harper Jan 17, 2024
cf8b604
adding suffix to append to external ids as a parameter
michael-harper Jan 17, 2024
52d7c3c
removing external id suffix properly
michael-harper Jan 17, 2024
1037931
using dataclass to retriev gvcf path from dictionary
michael-harper Jan 17, 2024
3295307
not using sg= inside a list as this is not allowed
michael-harper Jan 17, 2024
ebe2689
removing project_id as parameter input
michael-harper Jan 17, 2024
5239ebd
adding print to sg for debugging
michael-harper Jan 17, 2024
6c07839
was iterating over "test" project to fetch data instead of "main" pro…
michael-harper Jan 17, 2024
a3839ad
Merge branch 'dev' into populate-tob-test-with-nagim
michael-harper Jan 17, 2024
315bc0a
correct assay["type"] assignment
michael-harper Jan 17, 2024
0299f7e
changed naming of project-id-to-add-to parameter. Shortened it to pro…
michael-harper Jan 17, 2024
94c7cc5
linting issues!
michael-harper Jan 17, 2024
64310cf
Update scripts/add_nagim_gvcfs_to_tob_wgs_test_metamist.py
michael-harper Jan 18, 2024
4df08e1
Update scripts/add_nagim_gvcfs_to_tob_wgs_test_metamist.py
michael-harper Jan 18, 2024
2f66f18
fixing referencing of sequencing group attributes when upserting assay
michael-harper Jan 18, 2024
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192 changes: 192 additions & 0 deletions scripts/add_nagim_gvcfs_to_tob_wgs_test_metamist.py
Original file line number Diff line number Diff line change
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import csv
from dataclasses import dataclass
from pprint import pprint

import click
from cpg_utils import to_path

from metamist.apis import ParticipantApi, AnalysisApi
from metamist.graphql import gql, query
from metamist.models import (
ParticipantUpsert,
SampleUpsert,
SequencingGroupUpsert,
AssayUpsert,
AnalysisStatus,
Analysis,
)


@dataclass
class RowData:
"""
A class used to represent a row of data.

Attributes
----------
sgid : str
an identifier for the sequencing group
ext_id : str
an external identifier for the row
gvcf : str
the path to the gvcf file
gvcf_idx : str
the path to the gvcf index file
"""

sgid: str
ext_id: str
gvcf: str
gvcf_idx: str


PARTICIPANT_QUERY = gql(
"""
query ($project: String!) {
project(name: $project) {
id
participants {
externalId
id
samples {
id
externalId
sequencingGroups {
externalIds
id
meta
platform
technology
type
assays {
meta
id
}
}
}
}
}
}
"""
)


@click.command()
@click.option(
'--project',
required=True,
help='The name of the project to add samples to.',
)
@click.option(
'--sample-path-mappings',
required=True,
help="""The path to a CSV file containing mappings of `main` CPG ID's,
the `external_id` and `gvcf` paths.
The file should have at least four columns: sgid, ext_id, gvcf, and gvcf_idx.
Here's an example of what the first couple of lines might look like:

sgid,ext_id,gvcf,gvcf_idx
sg1,ext1,gvcf1,gvcf_idx1
sg2,ext2,gvcf2,gvcf_idx2
""",
)
@click.option(
'--project-id',
required=True,
type=int,
help="""The ID of the project to add samples to.
For example: iterate over `main` project (identified by the --project flag) to get data for each sample, then create a new participant
with the same data, but with a new external ID that has the suffix (--suffix) specified by the user.
Then upsert these into the `test` project.
""",
)
@click.option(
'--suffix',
required=True,
help="""The suffix to add to the external ID's of the participants.
For example, if the suffix is `test`, then the external ID's of the participants
will be `ext_id1-test`, `ext_id2-test`, etc.
""",
)
def main(project: str, project_id: int, sample_path_mappings: str, suffix: str):
"""
Iterate over `main` project to get data for each sample, then create a new participant
with the same data, but with a new external ID that has the suffix specified by the user.
Then upsert these into the `test` project.
"""
# Read the CSV file into a dictionary
ext_id_to_row = {}
with to_path(sample_path_mappings).open() as f:
reader = csv.reader(f)
next(reader) # skip the header
for row in reader:
data = RowData(*row[:4])
ext_id_to_row[data.ext_id] = data

query_response = query(PARTICIPANT_QUERY, {'project': project})
p_upserts = []
# pylint: disable=unsubscriptable-object
for participant in query_response['project']['participants']:
if participant['externalId'] not in ext_id_to_row:
continue
ext_id = f"{participant['externalId']}-{suffix}"
p = ParticipantUpsert(
external_id=ext_id,
active=None,
samples=[],
)
for sample in participant['samples']:
s = SampleUpsert(
external_id=ext_id,
project=project_id,
sequencing_groups=[],
)
for sg in sample['sequencingGroups']:
pprint(sg)
s.sequencing_groups.append(
SequencingGroupUpsert(
type=sg['type'],
technology=sg['technology'],
platform=sg['platform'],
meta=None,
sample_id=None,
external_ids=None,
assays=[
AssayUpsert(
type=sg['type'],
meta={
'sequencing_type': sg['sequencing_type'],
'sequencing_platform': sg['sequencing_platform'],
'sequencing_technology': sg[
'sequencing_technology'
],
},
),
],
)
)
p.samples.append(s)
p_upserts.append(p)
# pylint: enable=unsubscriptable-object

upserted_participants = ParticipantApi().upsert_participants(project, p_upserts)

for participant in upserted_participants:
for sample in participant['samples']:
old_ext_id = sample['externalId'].removesuffix(f'-{suffix}')
row_data = ext_id_to_row[old_ext_id]
gvcf_path = row_data.gvcf # get gvcf path from dictionary
AnalysisApi().create_analysis(
project,
Analysis(
type='gvcf',
status=AnalysisStatus('completed'),
output=gvcf_path,
sequencing_group_id=sample['sequencingGroups'][0]['id'],
active=True,
),
)


if __name__ == '__main__':
main() # pylint: disable=no-value-for-parameter
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