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MichaelClerx authored and chonlei committed Mar 25, 2020
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Expand Up @@ -4,7 +4,9 @@ A detailed mathematical modelling of voltage-clamp experiment.
This repo contains all data and code for reproducing the results in the paper "*Accounting for variability in ion current recordings using a mathematical model of artefacts in voltage-clamp experiments*" by Chon Lok Lei, Michael Clerx, Dominic Whittaker, David Gavaghan, Teun de Boer, and Gary Mirams.


### Prerequisite
### Installing

To run the code to generate the data and figures shown in the paper, please follow these steps first:

1. Clone [hERGRapidCharacterisation](https://github.com/CardiacModelling/hERGRapidCharacterisation) to the relative path `../hERGRapidCharacterisation`.
2. To setup, either run (for Linux/macOS users)
Expand All @@ -18,29 +20,29 @@ install [PINTS](https://github.com/pints-team/pints) and [Myokit](http://myokit.
### Results

#### Section 3: Validating the mathematical model with electrical model cell experiments
[model-cell-experiments](./model-cell-experiments): Application of the voltage-clamp experiment model to electrical model cell experiments.

It contains results and code to reproduce Figures 4, 5; Table 2; Figures S4, S5; Tables S1, S2.
- [model-cell-experiments](./model-cell-experiments): Application of the voltage-clamp experiment model to electrical model cell experiments.
- contains results and code to reproduce Figures 4, 5; Table 2; Figures S4, S5; Tables S1, S2.

#### Section 4: Application to variability in CHO-hERG1a patch-clamp data
[herg-syn-study](./herg-syn-study): Synthetic studies with the voltage-clamp experiment model.

It contains results and code to reproduce Figure S6.
- [herg-syn-study](./herg-syn-study): Synthetic studies with the voltage-clamp experiment model.
- contains results and code to reproduce Figure S6.

[herg-real-data](./herg-real-data): Application of the voltage-clamp experiment model to data in [hERGRapidCharacterisation](https://github.com/CardiacModelling/hERGRapidCharacterisation).
- [herg-real-data](./herg-real-data): Application of the voltage-clamp experiment model to data in [hERGRapidCharacterisation](https://github.com/CardiacModelling/hERGRapidCharacterisation).

It contains results and code to reproduce Hypothesis 2: Figures 7, 8; Figures S7, S8, S9; Tables S3, S4.
It also contains results and code that are mentioned but not shown in the paper: full voltage-clamp experiment model fitting to hERG data; independent kinetics with independent artefacts 'Hypothesis 3'.
- Contains results and code to reproduce Hypothesis 2: Figures 7, 8; Figures S7, S8, S9; Tables S3, S4.
- Also contains results and code that are mentioned but not shown in the paper: full voltage-clamp experiment model fitting to hERG data; independent kinetics with independent artefacts 'Hypothesis 3'.

### Supporting files

[lib](./lib): Contains all modules/utility functions.
- [lib](./lib): Contains all modules/utility functions.

[mmt-model-files](./mmt-model-files): [Myokit](http://myokit.org/) model files, contains IKr model and voltage clamp experiment model etc.
- [mmt-model-files](./mmt-model-files): [Myokit](http://myokit.org/) model files, contains IKr model and voltage clamp experiment model etc.

[protocol-time-series](./protocol-time-series): Contains protocols as time series, stored as `.csv` files, with time points (in `s`) and voltage (in `mV`)
- [protocol-time-series](./protocol-time-series): Contains protocols as time series, stored as `.csv` files, with time points (in `s`) and voltage (in `mV`)

`data`, `data-autoLC`, `manualselection`, `qc`: symbolic links to [hERGRapidCharacterisation](https://github.com/CardiacModelling/hERGRapidCharacterisation)
- `data`, `data-autoLC`, `manualselection`, `qc`: symbolic links to [hERGRapidCharacterisation](https://github.com/CardiacModelling/hERGRapidCharacterisation)


## Acknowledging this work
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