Louis 354

• Male brown mouse

• G6f in layer 2/3

• DOB 11-9-14

• Implanted with 5 mm implants on 2-13-15

• Cage : 3639678 from 3607290, 3559448

• Experimental days

  • 07/07/2015 : Mouse was given 1 block all go trials for 20 min, no lick necessary and 2 blocks of 20 min of go trials with lick necessary for reward delivery. Because of software, behavior traces were not automatically saved but saved in a different file called LostTraces. First mouse on the setup in this experiment run.

  • 07/08/2015 : Mouse was given 2 blocks of 30 min with all go trials, lick necessary. It did well, with more than 350 responded trials.

  • 07/09/2015 : Mouse was given 2 blocks of 30 min with go+no go trials 100 % contrasts with air puff and time out on no go trials. Mouse is obviously confused by the change. For second block, we increase time out from 3s to 8s. As it was advantageous for him to lick timing wise. Block 1: Hits 72% FA 62 % Block 2: Hits 54% FA 46 %

  • 07/10/2015 : Mouse was given 2 blocks of 30 min with go+no go trials 100 % contrasts with air puff and time out on no go trials.
    Block 1: Hits 90% FA 88 %
    Block 2: Hits 47% FA 30 %

  • 07/11/2015: Tested the lick detector (ok). Mouse was given 2 blocks of 30 min trials with go + n ogo 100% contrasts with air puff and time out on no go trials.
    Block 1: Hits 99 % FA 94 %
    Block 2: Hits 98 % FA 31 % !!!!

  • 07/12/2015: Tested the lick detector (ok). Mouse was given 2 blocks of 30 min trials with go + nogo 100% contrasts with air puff and time out on no go trials.
    Block 1: Hits 99 % FA 7 %
    Block 2: Hits 35 % FA 2 % losing interest in water but discriminate

  • 07/13/2015: Tested the lick detector (ok). Mouse was given 2 blocks of 30 min trials with go + nogo 100% contrasts with air puff and time out on no go trials.
    Block 1: Hits 97 % FA 33 %
    Block 2: Hits 97 % FA 2 %

  • 07/14/2015: Tested the lick detector (ok). Mouse was given 2 blocks of 30 min trials with go + nogo 100% contrasts with air puff and time out on no go trials.
    Delay was increased to -1 in first block. Delay was 0 in the second block Block 1: Hits 98 % FA 12 %
    Block 2: Hits 99 % FA 3 %

  • 07/15/2015 We started the day by giving the mouse trials with go + no go 100% contrast with air puff and time out on no go trials. The reward delay was set to 0.5 sec. In the very first Go trials, the mouse licked only during the presentation of the stimulus and was thus not given the reward. Afterwards, he didn't lick at all. For a couple of trials, we gave him manual rewards after the response cue during the go trials, and left the mouse to figure out the new rule. His strategy is to stop licking rather than explore and be punished.Because after ~12 min the mouse is not responding, we stopped the block and gave him 5 min of the same trial structure, with the exception that Go trials did not need a lick before reward delivery. We were hoping that the mouse will understand that the reward comes 0.5 sec after the go stimulus, and that licking during Go stimulus is not necessary. After this 5 min session, we gave the mouse 5 min session with the Go trials needing a lick (so the same trial structure we tried at the beginning). Show block 1 (5 min) : Hits 96% FA 0%
    Test block 1 (5 min) : Hits 29%; FA 0%
    Show block 2 (5 min) : Hits 100% FA 7%
    Test block 2 (5 min) : Hits 0% FA 0%
    Test block 3 (5 min) : Hits 0% FA 0%
    Show block 3 (5 min) : Hits 95% FA 19%
    Test block 4 (2 min) : Hits 0% FA 0%
    Show block 4 (2 min) : Hits FA
    Test block 5 (2 min) : Hits 0% FA 0%
    Show block 5 (2 min) : Hits 100% FA 12%
    Test block 6 (2 min) : Hits 0% FA 0%
    Test block 7 (5 min) Go stimulus + lick, Go only, Reward delay 0 : Hits 0%
    Test block 7 (5 min) Go stimulus + lick, Go only, Reward delay 0 : Hits 0%
    Test block 7 (1 min) Go stimulus + lick, Go only, Reward delay 0 : Hits 0%
    Test block 7 (5 min) Go stimulus, Go only, Reward delay 0 : Hits 100%
    Test block 7 (5 min) Go stimulus + lick, Go only, Reward delay 0 : Hits 52 %
    Test block 7 (5 min) Go stimulus + lick, Go + no go, Reward delay 0 : Hits 18% FA 0

  • 07/16/2015: Tested the lick detector (ok). Mouse was given 1 blocks of 10 min trials with go + nogo 100% contrasts with air puff and time out on no go trials. Second block is 30 min, the same Delay was-1 in first block. third block is 0s delay with 10 min Block 1: Hits 57 % FA 10 %
    Block 2: Hits 100 % FA 12 %
    Block 3: Hits 100 % FA 0 %

  • 07/17/2015: tested the lick detector (ok). 1 5 min block go +nogo 100% contrast, air puff and TO on no go trials. reward delay 0 sec, mouse didn't respond at all to go cues.test lick detector (ok). block 2, 20 min go +nogo 100% contrast, air puff and TO on no go trials. reward delay -1 sec. Mouse initially didn't respond and gave him a few manual rewards. He started responding afterwards. Block 3 20 min go +no go 100% contrast, air puff and TO on no go trials. reward delay 0 sec. Mouse initially not responding. Gave him a few manual rewards, still not responding. block 4, 20 min go +nogo 100% contrast, air puff and TO on no go trials. reward delay -1 sec. Mouse only starts responding after a few manual rewards, then stops responding. Behavior is not robust.
    Block 1 Hit 0% FA 0%
    Block 2 Hit 86% FA 9%
    Block 3 Hit 15 % FA 0%
    Block 4 Hit 10 % FA 1% at the end of the day, mouse had another 30 min, go + no go, no go punished by TO and air puff, reward delay -1. mouse again licked very little and in epochs.
    Block 5 Hit 44 % FA 0%

  • 07/18/2015: tested the lick detector (ok). 1 20 min block go +nogo 100% contrast, air puff and TO on no go trials. reward delay -1 sec. behavior robust. block 2, 20 min go +nogo 100% contrast, air puff and TO on no go trials. reward delay 0 sec. Interrupted and restarted block 2 to give some water reward. Mouse was just asleep Mouse clearly is good but has a short attention span. block 3 is 0.5s. Mouse is doing it but is going in and out of the task. Block 1 Hit 76% FA 1%
    Block 2 Hit 59% FA 1%
    Block 3 Hit 6 % FA 1%

  • 07/19/2015 tested the lick detector (ok). 1 20 min go +nogo 100% contrast, air puff and TO on no go trials. reward delay 0 sec. Mouse makes no attempt to lick. Gave him two manual rewards, then he licked on the next couple trials. Mouse not engaged at all in the task. Block 2. Increased the reward valve duration from 0.06 to 0.1 in case the reward is not motivating enough for the mouse. doesn't work. Gave the mouse rewardsa for a couple of minutes (tested the lick detector ok). Block 3 30 min go +nogo 100% contrast, air puff and TO on no go trials. reward delay 0 sec.reward valve duration 0 sec
    Block 1 Hit 10 % FA % (2 out of 3 rewards were manually delivered)
    Block 2 Hit 2 % FA 1% mouse makes no attempt to lick. Moved the reward valve duration back to 0.06 Block 3 Hit 0 % FA %

  • 07/20/2015 tested the lick detector (ok). 1 20 min go +nogo 100% contrast, air puff and TO on no go trials. reward delay 0.5 sec. Block 1 mouse forgot lick is necessary. delay is 0.5. We tried to engage manually multiple times but he never went for it by himself. Block 2 only go with delay 0s. block 3 only go with delay -2. This mouse has got out. We need to start again. Block 1 Hit 0 % FA %
    Block 2 Hit 0 % FA %
    Block 3 Hit % FA %
    In the afternoon. BLock 4 go + lick required + -2 second reward delay; block 5, 20 min Go no go, no go punished by air puff and TO, reward delay -2 sec and 0.1 duration Block 4 Hits 100% Block 5 Hits 52% FA 11%

  • 07/21/2015 block 1,2,3 : 20 min go +nogo 100% contrast, air puff and TO on no go trials. reward delay -2 sec. reward 0.06 duration
    Block 1 Hit 98 % FA 38 %
    Block 2 Hit 84 % FA 6 %
    Block 3 Hit 24 % FA 2 %

  • 07/22/2015 tested lick detector (ok). 3 20 min blocks. first block, go +nogo 100% contrast, air puff and TO on no go trials. reward delay -2 sec. reward 0.06 duration. second block, reward delay -1 sec. third block, reward delay 0s. interrupted as mouse zoned out. Gave a few reward and start again. Block 1: Hit 96 % FA 17 %
    Block 2: Hit 98 % FA 7 % Block 3: zoning out, interrupted Block 4: again Hit 42 % FA 0 %

  • 07/23/2015 tested lick detector (ok). first 20 min block, go +nogo 100% contrast, air puff and TO on no go trials. reward delay 0 sec. reward 0.06 duration. Mouse doesn't lick at all. gave him some free reward and he still doesn't respond. we take mouse out and run him again at the end of the day. 20 min block, go +nogo 100% contrast, air puff and TO on no go trials. reward delay 0 sec. reward 0.06 duration. he's doing perfectly fine in the afternoon. Block 2, reward delay 0.5 sec, block 3, the same, to consolidate the behavior Block 1 Hits 99% FA 11%
    Block 2 Hits 100% FA 8%
    Block 3 Hits 96 % FA 5 %

  • 07/24/2015 gave mouse some free rewards. first 20 min block, go +nogo 100% contrast, air puff and TO on no go trials. reward delay 0.5 sec. reward 0.06 duration.mouse doesn't lick at all. stopped the block, gave him more free rewards and started again. Mouse doesn't lick at all. Again, did a few trials with Go only, no lick necessary. Mouse licked immediately after those trials but dropped off immediately. After another 10 min of no licking, I took him out to run him tonight again.

  • 07/25/2015 gave mouse some free rewards. initially he didn't even drink the water. eventually he drank the water but did not engage in the task. he had a couple of more free rewards before making any response and engaging in the task. 3 20 min blocks. first & second 20 min block, go +nogo 100% contrast, air puff and TO on no go trials. reward delay 0.5 sec. reward 0.06 duration. during the second block, the mouse briefly stopped responding but started again after a few free rewards. third block, introduced air puff punishments for random licks. the mouse licks almost always only at the tone, he's very good at holding off licks.towards the end of the third block he was a bit confused about when to lick in go trials.
    Block 1: Hit 96% FA 18% Block 2: hit 85 % FA 3%
    Block 3: Hits 57% FA 4% air puffs 59 (random lick punished by air puff for first time)

  • 07/26/2015 I had to "boot up" his behavior. First I gave some free reward. THan I gave some free reward during go trials with the right temporal structure. Then I asked him to lick with only go Trials and then I introduced the no go trial again. random licks with air puff for block 1 and 2. Quickly rebooted again between block1 and 2 with few free rewards and a few trials of only go. This mouse needs to be started apparently to reactivate its context. Time out for block 3. Mouse is "Extremely" good at holding its lick. It found a way to move its jaw during the visual stimulus so as to maintain its response but not licking. Very interesting behavior. It's VERY important not to have any delays between blocks as it seems this mouse need time to get into the behavior. Block 1: Hit 94 % FA 7 % trials 158 air puffs 184 Block 2: hit 96 % FA 5 % trials 159 air puffs 36
    Block 3: Hits 72 % FA 0 % trials 159 air puffs 8 Mouse phased out toward the end.

  • 07/27/2015 I had to "boot up" his behavior. First I gave some free reward. THan I gave some free reward during go trials with the right temporal structure. Block 1 as before. Block 2 time out during lick moved to 3s. Block 1: Hit % FA % trials air puffs
    Block 1,5 : Interrupted in the middle by touching keyboard
    Block 2: hit 100 % FA 2.6 % trials 148 air puffs 31
    Block 3: Hits 81 % FA 1.4 % trials 151 air puffs 23 phasing out in the end

  • 07/28/2015 Mouse is performing perfectly but lose interest very quickly. We move contrast to 50 % and mouse was doing as well. Data is distributed in small block as we tried to reengage him multiple times so it's hard to show summary stat here but similar to yesterday except less reliably engaged.
    Mapping: 0 degrees (bar going top to bottom) and 90 degrees (bar going front to back of the mouse in left eye) Filenames: L354Mapping0deg and L354Mapping90deg Duration 1600 seconds each (102 trials each). Behavioral parameters are in a Mosaic file. 90 deg recording was ~24 minutes, but behavioral traces were not recorded.

• 07/29/2015 wake up protocol: Go only, no lick necessary, Go only, lick necessary, then full task.Go+nogo, no go punished by TO and air puff, random lick punished by air puff and 3 sec TO, rewards valve duration 0.06, reward delay 0.5 sec. Block 1, contrast is 50%. Block 1: Hit 60% FA 11% air puff 16 trials 154
  Block 2: Hit 27% FA 1% air puff 11 trials 154

• 07/30/2015 wake up protocol: Go only, no lick necessary, Go only, lick necessary. Didn't work in the morning. try again in the afternoon. Mouse was hard to start in the afternoon but eventually got there. initially, the mouse had small 5 min blocks before attention waned
  Block 1: Hit 94% FA 8% air puff 12 trials 156 Block 2: Hit FA air puff trials contrast dropped to 25%. mouse didn't respond

• 07/31/2015 wake up protocol: Go only, no lick necessary, Go only, lick necessary.Mouse responded. Mouse stopped responding when adding No go trials, so gave mouse a few go+nogo trials, no lick necessary for go; mouse started responding after that on the full task, 25% contrast. mouse stopped responding 5 min into the block
  Block 1: Hit 34% FA 4% air puff 8 trials 136 try again in the evening. in the evening, did not do any wake up protocols
  Block 1: Hit 56% FA 17% air puffs 21 trials 152
  Block 2: Hit 58% FA 17% air puff 16 trials 153
  Block 3: Hit 49% FA 11% air puff 11 trials 156

• 08/01/2015 full task, 25% contrast, no "wake up" protocol. In block 1, mouse started responding on his own ~12 min in. Mouse zoned out last 4 min of block 2 and did not reengage the task during block 3 until, again, ~12 min in
  Block 1: Hits 32% FA 7% air puffs 6 trials 158
  Block 2: Hits 67% FA 9% air puff 11 trials 156
  Block 3: Hits 40% FA 10% air puff 15 trials 154

• 08/02/2015 full task, 25% contrast, no "wake up" protocol. Mouse is doing extremely well (too well?). Block 1: Hits 37 % FA 3 % air puffs 5 trials 158 Block 2: Hits 84 % FA 10 % air puff 9 trials 157
  Block 3: Hits 84 % FA 8 % air puff 14 trials 155 Mapping (This mapping is actually for another mouse that was not imaged later, probably L363): 0 degrees (bar going top to bottom) and 90 degrees (bar going front to back of the mouse in left eye) Filenames: L354Mapping0deg and L354Mapping90deg Duration 1600 seconds each (102 trials each). Behavioral parameters are in a Mosaic file.

• 08/03/2015 full task, 25% contrast, no "wake up" protocol. First day Imaging. Recorded full behavior movie+behavior traces+calcium movie. Behavior is 1260 seconds long and calcium movie 1200 s so as to record the extra-frames Triggers sent by the camera on trigger 2 (about 20 extra TTLs). Block 1: Hits % FA % air puffs trials
  Block 2: Hits % FA % air puff trials
  Block 3: Hits % FA % air puff trials
  Mouse is performing really well. With Off periods at the beginning. Check the mouse behavior movie for dropped frames and proper sync with licking behavior.

• 08/04/2015 full task, 25% contrast, no "wake up" protocol. Imaging. Recorded full behavior movie+behavior traces+calcium movie. Block 1: Hits 44 % FA 5 % air puffs 6 trials 166 Mouse took some time to wake up and then also zoned out in the end.
  Block 2: Hits 85 % FA 7 % air puff 6 trials 166 Block 3: Hits 67 % FA 2 % air puff 10 trials 164

• 08/05/2015 full task, 25% contrast, no "wake up" protocol. Imaging. Recorded full behavior movie+behavior traces+calcium movie. Block 1: Hits 61 % FA 2.5 % air puffs 8 trials 165 Block 2: Hits 78 % FA 8 % air puff 14 trials 163 Block 3: Hits 68 % FA 2.5 % air puff 5 trials 166 Perfect data

• 08/06/2015 full task, , no "wake up" protocol. Imaging.
  Recorded full behavior movie+behavior traces+calcium movie.
  Block 1: Hits 71% FA 3 % air puffs 11 trials 164 25% contrast
  Block 2: Hits 76 % FA 10 % air puff 24 trials 159 12% contrast
  Block 3: Hits 71 % FA % 10 air puff 18 trials 161 12% contrast

• 08/07/2015 full task, , no "wake up" protocol. Imaging.
  Recorded full behavior movie+behavior traces+calcium movie.
  Block 1: Hits 30 % FA 8 % air puffs 45 trials 150 12% contrast
  Block 2: Hits 93 % FA 24 % air puff 40 trials 152 12% contrast
  Block 3: Hits 73 % FA % 17 air puff 15 trials 162 12% contrast Between block 1 and 2, I moved the lick tube a little away from the mouse, it seemed to be triggering artifactual licks with whiskers. TO BE CHECKED by looking at lick data and behavior movie closely.

• 08/08/2015 full task, , no "wake up" protocol. Imaging.
  Recorded full behavior movie+behavior traces+calcium movie. There is a big calcium cloud every time the mouse gets the reward in the center. This area was also pulsing very strongly as I was positioning the mouse on the holder. Block 1: Hits 87 % FA 29 % air puffs 32 trials 155 12% contrast
  Block 2: Hits 92 % FA 21 % air puff 27 trials 157 12% contrast
  Block 3: Hits 79% FA 17 % air puffs 19 trials 161 12% contrast

Perfect data

• Weight :

80 % of 30.8 = 24.6 g

Day	Weight pre-water	Weight post-behavior	Weight 1h later	Given water	Weight post-water
June 30	30.8 g			None
July 1	28.1 g			0.75 mL	28.8 g
July 2	26.7 g			0.75 mL	27.8 g
July 3	26.1 g			0.7 mL	26.9 g
July 4	25.5 g			0.7 mL	26.2 g
July 5	24.8 g			0.7 mL	25.4 g
July 6	23.9 g			0.7 mL	24.6 g
July 7	23.4 g	23.9 g		0.7 mL	24.5 g
July 8	23.5 g	25.1 g
July 9	24 g	24.8 g
July 10	23.8 g	24.5 g
July 11	23.6 g	24.8 g	24.8 g	0.5 ml	25.3 g
July 12	24.1 g	25 g
July 13	23.7 g	24.9 g
July 14	?? g	24.6 g
July 15	23.3 g	23.8 g
July 16	22.8 g	23.4 g	22.8	0.9	23.7
July 17	23.4 g	23.6 g
July 18	22.5 g	22.8 g	22.6	1	23.6
July 19	22.9 g	23.1 g
July 19	22.2 g	22.4 g	22.4		24.1
July 20	23.4 g	24.7 g
July 21	23.6 g	XXXX g
July 22	23.6 g	24.8 g
July 23	23.9 g	24 g (short session)	23.7 (late sess)		25.1
July 24	24.3 g	24.5 (short session)	24.5 (late sess)
July 25	23.2 g	24.7 g
July 26	23.3 g	24.8 g
July 27	23.7 g	24.9 g
July 28	23.9 g	24.7 g
July 29	23.4 g	24.2 g
July 30	23.3 g	23.7 g	23.2 (late sess)		24.3
July 31	23.5 g	23.9 g	23.7 (late sess)		24.2
August 1	23.6 g	24.2 g
August 2	23.1 g	23.9 g
August 3	22.9 g	g
August 4	23 g	23.5 g
August 5	22.9 g	23.6 g
August 6	g	23.5 g
August 7	22.7 g	23.6 g
August 7	22.7 g	23.7 g	END OF WATER DEPRIVATION